2012年12月1日 星期六

鳥苷三磷酸GTP ELISA套組。Guanosine Triphosphate (GTP) ELISA KIT, E96823


Guanosine Triphosphate (GTP) ELISA KIT, E96823
CAS86-01-1
USCN96823
WikiGTP
Guanosine-5'-triphosphate (GTP) is a purine nucleotide. It can act as a substrate for the synthesis of RNA during the transcription process. Its structure is similar to that of the guanine nucleobase, the only difference being that nucleotides like GTP have a ribose sugar and three phosphates, with the nucleobase attached to the 1' and the triphosphate moiety attached to the 5' carbons of the ribose.
It also has the role of a source of energy or an activator of substrates in metabolic reactions, like that of ATP, but more specific. It is used as a source of energy for protein synthesis.GTP is essential to signal transduction, particularly with G-proteins, in second-messenger mechanisms where it is converted to GDP (guanosine diphosphate) through the action of GTPases.
ELISA Kits(Enzyme-linked immunosorbent assay Kits)
Catalog Product Name Organism Manual
E96823GeELISA Kit for Guanosine Triphosphate (GTP)Generaln/a
CLIA Kits(Chemiluminescent immunoassay Kits)
Catalog Product Name Organism Manual
C96823GeCLIA Kit for Guanosine Triphosphate (GTP)Generaln/a
 
【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
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Uscnlife如何克服ELISA產品問題? 網站上有一個ELISA試劑盒的操作視頻，客戶可對照著進行操作，這樣可以避免操作原因引起的原因不可用。

如何克服ELISA問題；
標準曲線值較低而且不同濃度標準品之間沒有梯度。
要判斷原因，如下所示；
1. 試劑盒如何保存？
2. 是否按照說明書的要求，在臨用前配製各種反應試劑，如標準品溶液，檢測液A及檢測液B工作液？
3. 酶催化底物反應多長時間，即從加入TMB到加入終止液的時間
4. 溫育的反應溫度是37度，是使用恒溫箱溫育還是水浴？
5. 酶標儀讀數的波長是否設置於450nm？
在我們的網站上有一個ELISA試劑盒的操作視頻，客戶可對照著進行操作，這樣可以避免操作原因引起的原因不可用。
網站網址如下http://www.uscnk.cn/news/2010428143735.htm。

【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

 
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肝功能分析試劑, A型肝炎抗體 C型肝炎抗體、肝功能指數 (GOT)、肝功能指數 (GPT)

肝功能分析試劑
1. A型肝炎 (anti-HAV)
IT-004-011
Anti-VP1 (HAV)
Antibody
200ug/1mg
3. C型肝炎 (anti-HCV)
IT-004 -005M3
Anti-E2(HCV)
Antibody
100ug/500ug
4. B型肝炎抗原 (HBsAg)
IT-004-0011
Anti-HBsAg (Pre-S2 Specific) (HBV)
Antibody
5. B型肝炎抗體 (HBsAb)
IT-004-002Ep
PreS2-HBsAg(HBV/Genotype C)
Protein
100ug/1mg
6. 肝功能指數 (GOT)
K753-100
Aspartate Aminotransferase (AST or SGOT) Activity Assay Kit
100 assays
7. 肝功能指數 (GPT)
K752-100
Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit
8. 酒精性肝功能指數 (r-GTP)
9. 肝癌指標 (AFP)
10. 總蛋白指數 (TP)
11. 白蛋白指數 (Alb)
12. 球蛋白指數 (Glo)
13. 總蛋白指數 (T-Bil)
14. 直接膽紅素 (D-Bil)

【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
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IGF Like Family, Member 2 (IGFL2) ELISA酵素免疫分析套組 (Uscnlife ELISA KIT)台灣代理商

IGF Like Family, Member 2 (IGFL2) ELISA酵素免疫分析套組 (Uscnlife ELISA KIT)台灣代理商




IGFL2 belongs to the insulin-like growth factor (IGF) family of signaling molecules that play critical roles in cellular energy metabolism and in growth and development, especially prenatal growth .By EST database analysis and DNA sequence assembly, Emtage et al. (2006) identified 4 members of a human gene family, which they designated IGF-like (IGFL), encoding small secreted proteins.IGFL2 encodes a deduced 95-amino acid protein with a signal peptide followed by a conserved region with 11 regularly spaced cysteine residues, including 2 CC motifs. Real-time PCR detected IGFL 2 in cerebellum, heart, placenta, spleen, stomach, testis, and thymus. Emtage et al. (2006) identified only 1 Igfl sequence in mouse, which cannot be assigned direct orthology with any of the human IGFL



E81616HuELISA Kit for IGF Like Family, Member 2 (IGFL2)
E81616HuELISA Kit for IGF Like Family, Member 2 (IGFL2)




【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

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組蛋白去乙醯酶3（Histone Deacetylase 3）ELISA酵素免疫分析套組,HDAC3 Activity Assay Kit, K343-100

組蛋白去乙醯酶3（Histone Deacetylase 3）ELISA酵素免疫分析套組

HDAC3 Activity Assay Kit
Catalog#: K343-100 | Size: 100 assay.
Kit Summary:
• Detection method- Fluorescence (Em/Ex = 380/500 nm)
• Sample type- Cell and Tissue lysates, plasma and serum, other biological fluids
• Species reactivity- Mammalian
• Applications- Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-3 samples.
Features & Benefits:
• Simple procedure; takes ~ 60 min
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of HDAC3 activity
Kit Components:
• HDAC3 Assay Buffer
• HDAC3 Substrate
• HDAC3 Positive Control
• AFC Standard (1 mM)
• Developer
• Trichostatin A (10 µM)
Description:
Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-3 activity Kit, HDAC-3 and Developer will deacetylate and cleave the substrate [R-H-K-K(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples.
Storage Conditions:
-20°C (Upon receipt place HDAC-3 positive control at -80 °C for storage.)
Shipping Conditions:
gel pack

【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
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【GST Tag ELISA 檢測試劑盒】靈敏度高1 ng/ml GST標籤蛋白測量，快速，約1小時完成檢測


谷胱甘肽S-轉移酶標籤（GST tag）ELISA檢測試劑盒

靈敏度高1 ng/ml GST標籤蛋白，快速，約1小時完成檢測
用於鑑定細胞裂解物或者純化後的GST標籤蛋白，檢測蛋白的表達，以及蛋白表達優化過程中的快速測定。該試劑盒也可用於檢測GST標籤在標籤切除和蛋白純化後的殘留情況。

實驗原理


本試劑盒應用固相雙抗體夾心酶聯免疫檢測原理測定樣品GST標籤蛋白的含量。用純化的抗GST標籤的單克隆抗體包被微孔板，製成固相抗體，往包被單抗的微孔中依次加入GST 標籤蛋白樣品和GST 標籤蛋白標準樣，再與Biotin標記的另一個抗GST標籤的單克隆抗體結合。再加入HRP標記的Streptavidin形成抗體-抗原-酶標抗體複合物，經過清洗後加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，並在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的標籤蛋白的量呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品標籤蛋白濃度。

主要特點


靈敏度：1 ng/mL GST標籤蛋白
線性範圍：3.125~100 ng/mL
檢測樣品：細胞裂解液，純化後的GST標籤蛋白等
操作時間：快速，約1小時完成檢測
操作簡單：僅需要簡單的樣品處理就可以進行檢測
品質穩定：嚴格的生產工藝保證了批次間的穩定性

產品目錄


產品編號


產品名稱


規格


L00411


谷胱甘肽S-轉移酶標籤（GST tag）ELISA檢測試劑盒: (GST tag ELISA Detection Kit)


1Kit (96 assay)



【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
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【人類TTR ELISA套組】USCNLIFE貨號# E90726Hu。測量範圍93.75-6,000pg/mL。靈敏度36.55pg/mL。

ELISA Kit for Transthyretin (TTR)

TBPA; PA; PLB; Prealbumin; PALB; Prealbumin Amyloidosis Type I; Transports Thyroxine and Retinol

PDF TTR ELISA套組操作手冊下載, 貨號# E90726Hu


測量範圍


93.75-6,000pg/mL


靈敏度


36.55pg/mL



TTR was originally called prealbumin because it ran faster than albumins on electrophoresis gels.Prealbumin is produced by the choroid plexus, by pancreatic islet cells in the embryonic yolk sac, and by enterochromaffin cells in the gastrointestinal mucosa, but the liver is quantitatively the most important source.9 Liver production is maintained until late in liver disease.
Hydration status does not affect prealbumin levels. A negative acute phase reactant, the prealbumin level will transiently decrease in the presence of inflammation and in the immediate postsurgical period. Serum levels also decline in patients with conditions associated with protein malnutrition, such as malignancy, cirrhosis, protein-losing enteropathy, and zinc deficiency.


【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

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